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Mgbochi mgbochi TYPHOID. 69
ewepụrụ ya n'ụzọ aseptic. N'ụzọ yiri iko mkpanaka nke na-egbochi ndị kasị ala nke atọ mpụta tubes nke Ịgwakọta ite na-wepụrụ. Emere nke a, a na-ejikọta njikọ, site na otu iko iko igba ogwu, guzobe n'etiti tubes abụọ ahụ, a na-ewepụ ihe nkedo ahụ, mmiri ahụ na-apụkwa site na cultivation flask n'ime ite agwakọta. Ozugbo ego ole nke mmiri abanyela n'ime ite ngwakọta, na n'oge ọ bụla tupu mmiri ahụ enwee ruru n'ọkwa nke elu mpụta tube, nrụgide tubing nke bụ etinyere na tube n'akụkụ a na-adọta nwayọọ. Nke a na-akpata eriri waya nke bụ gburugburu njedebe capillary nke temometa paraffin iji wepụ nke a site na a ọrụ guillotine. Igwe ọkụ ọkụ a tọhapụrụ wee tụba n'ime mmiri ahụ, dị njikere maka ojiji. A na-ejide waya brass nke mezuru ụlọ ọrụ ya ugbu a na a ike, ma wepụ ya na tube.
Mgbe ọdịnaya nke cultivation flask gafere n'ime ịgwakọta ite, a na-edochi mwekota nke ite agwakọta n'elu tube roba n'ebe dị nso na oghere iko nke jikọtara akụkụ abụọ ahụ. Nke agịga nke sirinji nke jupụtara na carbolic acid na-agbasaghị bụ wee tinye n'ime lumen nke tube n'ebe dị n'etiti mwekota na iko nozzle nke e zoro aka na ya n'elu. Ihe sirinji nke carbolic acid a na-ebuba ya n'ime tube n'ime ite ihe ọkụkụ. Nke a mere, na Enwere ike ịpụpụ flask cultivation na-enweghị ihe egwu. Flask ihe ọkụkụ na-esote na usoro a na-ewere ugbu a n'aka n'otu ụzọ ahụ. Mgbe usoro nke na-ejuputa n'ime ite mix e dechara akụkụ tubes na-egbochi iberibe igba ogwu iko mkpanaka. A na-eji ngwọta rọba luted ndị a.
Ihe na-esote bụ ịnyefe ite ma ọ bụ ite ngwakọta na mmiri ịsa ahụ. A ga-eme nke a n'egbughị oge, a ga-ebukwa ite ndị dị otú ahụ n'ụzọ dị ka zere niile splashing elu nke omenala na n'akụkụ na olu nke ite ahụ. Mmiri ịsa ahụ aghaghị ịdị omimi nke ọma iji kwe ka mmiri na-abịa nke ọma n'ubu nke ite ngwakọta. Nke ikpeazụ a agaghị ejupụta n'ime 2 sentimita asatọ nke ọkwa a. Ọ bụrụ na ite ahụ, mgbe a na-ewebata ya n'ime mmiri ịsa ahụ, bụ chọpụtara na ọ na-amagharị, a na-arịda ya site n'ịfefe olu akwa led dị arọ n'olu ya. A na-etinye ite ndị ahụ n'enweghị ihe ọ bụla, ọ na-ekpo ọkụ etinyere na mmiri ịsa ahụ, na ọnọdụ nke thermometers na A na-ahụ ite dị iche iche site n'oge ruo n'oge. A na-eme nke ọma site n'iwetu a pkce nke ugogbe anya nkịtị n'okpuru mmiri dị n'ime mmiri ịsa ahụ. A na-atụgharị gas ahụ ozugbo temometa paraffin dara. Ite ogwu ogwu na-anọgide n'ime mmiri ọkụ maka nkeji 10 ruo 15 ọzọ.
Nzọụkwụ ọzọ bụ ịgwakọta ọdịnaya nke dum usoro agwakọta ite ka e mesịa nweta maka standardization a nnọchiteanya sample nke dum ime biya nke ogwu. A na-eme ngwakọta ahụ site na ijikọ ihe agwakọta ite na ibe ha site na ha rọba tubes (Fig. 2). Ndị a tubes rọba dị ogologo nke na-ekwe ka nke ọ bụla n'ime ite ahụ nọrọ n'efu ewelitere n'elu ma ọ bụ wedata n'okpuru ọkwa nke ite ibe ya. Site na ndị a pụtara ngwakọta nke ọdịnaya na-eme ngwa ngwa. N'ọnọdụ ebe ite ndị ahụ na-agwakọta juru eju nke na-etinye ihe isi ike n'ụzọ nke afọ ojuju Ngwakọta nke ihe dị n'ime ha, ite ọzọ na-ekpo ọkụ na-ekpo ọkụ bụ intro- dugara n'ime sekit. Ngwakọta nke emechara nke ọma, ihe atụ nke A na-ewepụ ọgwụ mgbochi na mauntr akọwara n'elu n'ihe metụtara
